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kapa stranded rna seq library prep kit  (Roche)


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    Roche kapa stranded rna seq library prep kit
    Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA <t>sequences.</t> <t>RNA-seq</t> was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.
    Kapa Stranded Rna Seq Library Prep Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+library+prep+kits/KAPA+RNA+Kit/pmc12992974-437-25-31
    Average 99 stars, based on 1 article reviews
    kapa stranded rna seq library prep kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury"

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.056

    Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA sequences. RNA-seq was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.
    Figure Legend Snippet: Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA sequences. RNA-seq was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.

    Techniques Used: Expressing, RNA Sequencing, Quantitative Proteomics

    Related Articles

    Next-Generation Sequencing:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Magnetic Beads:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Purification:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Software:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Sequencing:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    cDNA Library Assay:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Library Quantification:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Polymerase Chain Reaction:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Concentration Assay:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Control:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    RNA HS Assay:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Real-time Polymerase Chain Reaction:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Isolation:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Construct:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    RNA Sequencing Assay:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Sample Prep:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    RNA Detection:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    RNA Expression:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    RNA Extraction:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Methylation:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co

    Amplification:

    Article Title: Extracellular vesicle-mediated delivery of mitochondrial circRNA MTCO2 protects against cerebral ischemia by modulating mPTP-dependent ferroptosis ☆
    Article Snippet: dysregulation. Total RNA was extracted from mouse brain tissue by using RNAiso Plus (Takara Bio Inc., Japan). Ribosomal RNA was removed using KAPA RNA HyperPrep Kit with RiboErase for Illumina (HMR) (Kapa Biosystems, Inc., USA). PolyA tailing was performed on the rRNA-depleted RNA, followed by purification with Clean NGS magnetic beads (GC Biotech, the Netherlands). An RNase R reaction was then co



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    Image Search Results


    Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA sequences. RNA-seq was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.

    Journal: Bioactive Materials

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury

    doi: 10.1016/j.bioactmat.2026.02.056

    Figure Lengend Snippet: Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA sequences. RNA-seq was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.

    Article Snippet: For library construction, 1–2 μg of total RNA per sample was used. mRNA was enriched using oligo (dT) selection, and libraries were prepared using the KAPA Stranded RNA-Seq Library Prep Kit (Roche).

    Techniques: Expressing, RNA Sequencing, Quantitative Proteomics